ICAM1 antibody (FITC)

Catalog No. ABIN120497

The Mouse Monoclonal anti-ICAM1 antibody has been validated for FACS. It is suitable to detect ICAM1 in samples from Rat.

Quick Overview for ICAM1 antibody (FITC) (ABIN120497)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 1A29

Product Details anti-ICAM1 Antibody (FITC)

Specificity: This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend SM286LE for this use.

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein G

Immunogen: Rat Ax cells (a rat HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (Use 10 μL neat antibody to label 10^6 cells in 100 μL). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (see Ref.3). Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend the use of ABIN182014 for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL IgG

Buffer: PBS, pH 7.2, containing 1 % BSA as stabiliser and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54 / ICAM1

Background: CD54 cell surface antigen, also known as the intercellular adhesion molecule-1 (ICAM-1), is a 90 kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

Gene ID: 25464

NCBI Accession: NP_037099

UniProt: Q00238

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling